تعیین خصوصیات بیولوژیک و بیوشیمیایی سویه Pseudomonas fluorescens UTPF5 به‌عنوان یک باکتری پروبیوتیک گیاهی در ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی دانشگاه تهران

2 استاد گروه گیاهپزشکی دانشگاه تهران

3 استادیار دانشگاه رازی کرمانشاه

4 استادیار دانشگاه رفسنجان

5 دانش آموخته کارشناسی ارشد

چکیده

یافتن سویه­های جدیدی از باکتری­های پروبیوتیک گیاهی با قابلیت تجاری­سازی در کشاورزی از اهمیت زیادی برخوردار است. در این تحقیق، خصوصیات بیولوژیک و بیوشیمیایی سویه Pseudomonas fluorescens UTPF5 به‌عنوان یک باکتری مهم در ایران، مورد ارزیابی­ قرار گرفته است. تولید متابولیت­های مختلف از جمله اکسین، سیانید هیدروژن، سیدروفور، فنازین، دی استیل فلوروگلوسینول و تشکیل بیوفیلم و سیستم­های تنظیمی مورد بررسی قرار گرفت. مقدار بازدارندگی این سویه از رشد قارچ ‌Rhizoctonia solani تعیین شد. نتایج، تولید بسیاری از متابولیت‌های بیوکنترلی به استثناء فنازین را نشان داد. وجود آهن باعث کاهش تولید سیدروفور و افزایش سیانید هیدروژن شد. سویه UTPF5 قادر به تولید سیگنال، برای راه اندازی مسیر Gac/Rsm می‌باشد و اثر قابل توجهی در بیان hcnA و RsmZ دارد. این سویه قادر به تولید سیگنال هموسرین لاکتون نمی‌باشد. در بررسی‌های گلخانه‌‌ای توانایی بیوکنترل بیمارگر هدف و افزایش رشد گیاهان آفتابگردان، ارزن، لوبیا و کلزا به اثبات رسید.

 

کلیدواژه‌ها

موضوعات


Ahmadzadeh, M. 2014. Biological Control of Plant Diseases Plant Probiotic Bacteria (2th). University of Tehran Institute Publications (In Persian).
Alstrom, S. & Burns, R.G. 1989. Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition. Biology of Fertility Soil, 7: 232-238.
Alexander, D.B. & Zuberer, D.A. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria.  Biology of Fertility Soils, 12: 39-45.
Baker, K.F. 1987. Envolving concepts of biological control of plant pathogens. Annual Review of Phytopathology, 25: 67-85.
Benizri, E., Courtade, A., Picard, C. & Guckert, A. 1998. Role of maize root exudates in the production of auxin by Pseuomonas flurescens M.3.3. Soil Biology and Biochemestry, 30: 481-484.
Bora, T., Ozaktan, H., Gore, E. & Aslan, E. 2004. Biological control of Fusarium oxysporum f. sp. melonis by wettable powder formulations of the two strains of Pseudomonas putida. Journal of Phytophatology, 152: 471-475.
Branda, S.S., Ashild, V., Friedman, L. & Kolter, R. 2005. Biofilms: the matrix revisited. Microbiology, 13: 20-24.
Davey, M.E. & O Toole, G.A. 2000. Microbial biofilms: from ecology to molecular genetics. Microbial and Molecular Biology Reviews, 64: 847-867.
De Weert, S. & Bloemberg, G.V. 2006. Rhizosphere competence and the role of root colonization in biocontrol. Plant Associated Bacteria, 317-333.
Duffy, B.K. & De'fago, G. 1999. Environmental factors modulating antiobiotic and siderophore biosinthesis by Pseudomonas fluorescens biocontrol strains. Applied Environment Microbiology, 65: 2429-2438.
Edens, W.A., Goins, T.Q., Dooley, D. & Henson, J.M. 1999. Purification and characterization of a secreted laccase of Gaeumannomyces graminis var. tritici. Applied Environment Microbiology, 65: 3071-3074.
Farzane, M., 2008. Effect of antagonist bacterial in controlling Phytophthora cactorum crown and root rot of apple trees. MSc Thesis. University of Tehran (In Persian with English summary).
Fletcher, M. 1977. The effect of culture concentration and age, time and temperature on bacterial attachment to polystyrene. Canadian Journal of Microbiology, 23: 1-6.
Fukui, R., Poiner, E.I. & Schorth, M.N. 1991. Bacterial growth on inoculated sugar beet seed in soil and its effect in controlling infection by Pythium spp. (Abstr.) Phytopathology, 81:344.
Gordon, S.A. & Weber, R.P. 1950. Colorimetric estimation of indoleacetic acid. Plant Physiology, 30(1): 86–8.
Gottesman, S. & Stout, V. 2001. Regulation of capsular polysaccharide synthesis in Escherichia coli K-1. Molcular Microbiology, 5: 159-164.
Hagedorn, C., Gould, W.D. & Bradinelli, R.T. 1989. Rhizobacteria of cotton and their repression of seedling disease pathogens. Applied Environment Microbiology, 55: 2793-2797.
Kim, D.S., Cook, R.J. & Weller, D.M. 1997. Bacillus sp. L324-92 for biological control of tree root disease of wheat grown with reduced tillage. Phytopathology, 87: 551-558.
Lichstein, H.C. & Van De Sand, V.F. 2005. Violacin, an antibiotic pigment produced by Chromobacterium violaceum. Journal of Infect Disease, 55: 123-142.
Lugtenberg, B.J.J., Dekkers, L. & Bloemberg, G.V. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas. Annual Review of Phytopathology, 39: 461-490.
Maddula, V.S.R.K., Zhang, Z., Pierson, E.A. & Pierson, L.S. 2006. Quorom sensing and phenazines are involved in biofilm formation by Pseudomonas chlororaphis (aureofaciens) strain 30-84. Microbial Ecology, 52: 289-301.
Maurhofer, M., Keel, C., Hass, D. & Defago, G. 1995. Influence of plant species on disease suppression by Pseudomonas flourescens strain CHA0 with enhanced production. Plant Patology, 44: 40-50.
Maurhofer, M., Keel, C., Schnider, U., Voisard, C. & De'fago, G. 1992. Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity. Phytopathology, 82: 190-195.
Morris, C.E. & Monier, J.M. 2003. The ecological significance of biofilm formation by plant associated bacteria. Annual Review of Phytopathology, 41: 429-453.
Morrissey, J.P. & Osbourn, A.E. 1999. Fungal resistance to plant antibiotics as a mechanism of phatogenesis. Microbiology Molcular Biology Review, 63: 708- 724.
Nakkeeran, S., Dilantha Feenando, W.G. & Siddiqui, Z.A. 2005. Plant growth promoting rhizobacteria formulation and its scope in commercialization for the management of pests and disease. Z.A. Siddiqui (ed.), PGPR: Biocontrol and Bipfertilization, Springer, Dordrecht, the Netherland, 257-296.
Nandakumar, R., Babu, S., Viswanathan, R., Sheela, J., Raguchander, T. & Samiyappan, R. 2001. A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice biocontrol, 46: 493-510.
Notz, R., Maurhofer, M., Schnider–Keel, U., Duffy, B., Haas, D. & De'fago, G. 2001. Biotic factors affecting expression of the 2, 4 diacetylphloroglucinol biosynthesis gene phlA in Pseudomonas flourescens biocontrol strain CHA0 in the rhizosPhere. Phytopathology, 91: 873-881.
Oberhansli, T., Defago, G. & Hass, D. 1991. Indol-3-acetic acid (IAA) synthesis in the biocontrol strain CHA0 of Pseudomonas flourescens: role of tryptophan side chain oxidase. Journal of General Microbiology, 137: 2273-2276.
Picard, C.F.D., Cello, I., Ventura, M., Fani, R. & Guckert, A. 2000. Frequency and biodiversity of 2, 4-diacetylphloroglucinol production bacteria isolated from the maize rhizosphere at different stage of plant growth. Applied Environment Microbiology, 66: 948-955.
Sabaratnam, s. & Traquair, A.J. 2002. Formulation of a Streptomyces biocontrol agent for the suppression of Rhizictonia damping off in tomato transplants. Biological Control, 23: 245-253.
Schippers, B., Bakker, A.W. & Bakker, P.A.H.M. 1987. Interactions of deleterious and beneficial rhizosphere microorganism and the effect of cropping practices. Annual Review of Phytopathology, 25: 339-358.
Schisler, D.A., Slininger, P.J., Behle, R.W. & Jackson, M.A. 2004. Formulation of Bacillus spp. for biological control of plant disease. Phytophatology, 94: 1267-1271.
Sharifi, R., Ahmadzadeh, M., Sharifi-Tehrani, A. & Fallahzadeh, V. 2008. Competition for iron uptake by Pseudomonas fluorescens to control of Rhizoctonia solani Kuhen causing agent of bean damping off disease. Journal of Plant Protection, 22(2): 183-195
Siddiqui, I.A. & Shaukat, S.S. 2003. Suppression of root-knot disease by Pseudomonas fluorescens CHA0 in tomato: importance of bacterial secondary metabolite, 2, 4-diacetylpholoroglucinol. Soil Biology and Biochemistry, 35: 1615–1623.
Thomashow, L.S. & Weller, D.M. 1988. Role of a Phenazine Antibiotic from Pseudomonas fluorescens in Biological Control of Gaeumannomyces graminis var. tritici. Journal of Bacteriology, 170 (8): 3499-3508.
Vanetten, H., Temporini, E. & Wasman, C. 2001. Phytoalexin (and Phytoanticipin) tolerance as a virulence trait: Why is it not required by all pathogens? Physiology Molcular Plant Pathology, 59: 83-93.
Vincent, M.N., Harrison, L.A., Brackin, J., Kovacevich, P., Mukerji, P., Weller, D.M. & PIERSON, E.A. 1991. Genetic analysis of the antifungal activity of a soil borne Pseudomonas aureofacience strain. Applied Environment Microbiology, 48: 897-899.
Weller, D.M. 1988. Biological control of soil born plant pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology, 26: 379-407.
Weller, D.M. & Cook, R.J. 1983. Suppression of take all of wheat by seed treatment with fluorescent pseudomonadas. Phytophatology, 73: 463-469.
Yan, Z., Reddy, M.S. & Kloepper, J.W. 2003. Survival and colonization of rhizobacteria in tomato transplant system. Canadian Journal of Microbiology, 49: 383-389.