تعیین خصوصیات بیولوژیک و بیوشیمیایی سویه Pseudomonas fluorescens UTPF5 به‌عنوان یک باکتری پروبیوتیک گیاهی در ایران

نوع مقاله: مقاله علمی-پژوهشی

نویسندگان

1 گروه گیاهپزشکی دانشگاه تهران

2 استاد گروه گیاهپزشکی دانشگاه تهران

3 استادیار دانشگاه رازی کرمانشاه

4 استادیار دانشگاه رفسنجان

5 دانش آموخته کارشناسی ارشد

چکیده

یافتن سویه­های جدیدی از باکتری­های پروبیوتیک گیاهی با قابلیت تجاری­سازی در کشاورزی از اهمیت زیادی برخوردار است. در این تحقیق، خصوصیات بیولوژیک و بیوشیمیایی سویه Pseudomonas fluorescens UTPF5 به‌عنوان یک باکتری مهم در ایران، مورد ارزیابی­ قرار گرفته است. تولید متابولیت­های مختلف از جمله اکسین، سیانید هیدروژن، سیدروفور، فنازین، دی استیل فلوروگلوسینول و تشکیل بیوفیلم و سیستم­های تنظیمی مورد بررسی قرار گرفت. مقدار بازدارندگی این سویه از رشد قارچ ‌Rhizoctonia solani تعیین شد. نتایج، تولید بسیاری از متابولیت‌های بیوکنترلی به استثناء فنازین را نشان داد. وجود آهن باعث کاهش تولید سیدروفور و افزایش سیانید هیدروژن شد. سویه UTPF5 قادر به تولید سیگنال، برای راه اندازی مسیر Gac/Rsm می‌باشد و اثر قابل توجهی در بیان hcnA و RsmZ دارد. این سویه قادر به تولید سیگنال هموسرین لاکتون نمی‌باشد. در بررسی‌های گلخانه‌‌ای توانایی بیوکنترل بیمارگر هدف و افزایش رشد گیاهان آفتابگردان، ارزن، لوبیا و کلزا به اثبات رسید.

 

کلیدواژه‌ها

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